Project

Part:BBa_K1681000:Design

Designed by: Sameer Andani   Group: iGEM15_UIUC_Illinois   (2015-09-17)


iGEM standardized SCRIBE system from MIT's Lu Lab


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1267
    Illegal XhoI site found at 509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Gene for kanamycin resistance had to be mutated in the strain


Source

SCRIBE system from Dr. Lu's alb

References